Anticorps Recombinant de lapin anti-CPT2

CPT2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242381C5

N° de cat : 85013-4-PBS

Synonymes

242381C5, Carnitine O-palmitoyltransferase 2, mitochondrial, carnitine palmitoyltransferase 2, CPT II, CPTASE

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Galerie de données de validation

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  WB analysis using 85013-4-RR (same clone as 85013-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85013-4-RR (CPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85013-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85013-4-RR (same clone as 85013-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85013-4-RR (CPT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85013-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85013-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85013-4-RR against Human CPT2 were performed. The affinity constant is 0.261 nM.

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Informations sur le produit

85013-4-PBS cible CPT2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CPT2 Protéine recombinante Ag24897
• Nom complet: carnitine palmitoyltransferase 2
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002445
• Symbole du gène: CPT2
• Identification du gène (NCBI): 1376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).

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