Anticorps Monoclonal anti-CRABP2

• Phare
• Validé par KD/KO

CRABP2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A5F3

Avis (1)

N° de cat : 66468-1-Ig

Synonymes

1A5F3, Cellular retinoic acid binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II, CRABP II

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Galerie de données de validation

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  WB analysis of T-47D using 66468-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66468-1-Ig

  WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells.

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  WB analysis of MCF-7 using 66468-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 66468-1-Ig

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skin using 66468-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human skin cancer using 66468-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66468-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of MCF-7 using 66468-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules HEK-293, cellules MCF-7, tissu cutané de porc, tissu cutané de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer de la peau humain,
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66468-1-Ig cible CRABP2 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CRABP2 Protéine recombinante Ag0309
• Nom complet: cellular retinoic acid binding protein 2
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC001109
• Symbole du gène: CRABP2
• Identification du gène (NCBI): 1382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Publications

Species	Application	Title
mouse,human	IHC,IF	Cell Death Dis; Upregulation of CRABP2 by TET1-mediated DNA hydroxymethylation attenuates mitochondrial apoptosis and promotes oxaliplatin resistance in gastric cancer; Authors - Xiaolong Tang
human	WB,IHC,IF	Cell Signal; CRABP2 promotes peritoneal metastasis in CRC through TGF-β/Smad-mediated EMT signaling and invadopodia formation; Authors - Wende Hao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Linyi (Verified Customer) (01-17-2019)

We use this antibody on porcine chondrocytes lysate, 1/1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: chondrocytes, pig

CRABP2 Antibody Western Blot, validation (1/1000 dilution) in chondrocytes, pig (Cat no:66468-1-Ig)

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