Anticorps Polyclonal de lapin anti-CRB1

CRB1 Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26598-1-PBS

Synonymes

Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12

---

Galerie de données de validation

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB1 antibody (26598-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 26598-1-AP (same clone as 26598-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

26598-1-PBS cible CRB1 dans les applications de IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRB1 Protéine recombinante Ag24512
• Nom complet: crumbs homolog 1 (Drosophila)
• Masse moléculaire calculée: 1406 aa, 154 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC136271
• Symbole du gène: CRB1
• Identification du gène (NCBI): 23418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).

{{ptg:RelatedPrimaryAntibodies}}