Anticorps Polyclonal de lapin anti-CRBN

• Phare
• Validé par KD/KO

CRBN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 28494-1-AP

Synonymes

Protein cereblon, cereblon, AD-006

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Galerie de données de validation

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  WB analysis using 28494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 28494-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of COLO 320 using 28494-1-AP

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CRBN antibody (28494-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules 3T3-L1, cellules C6, cellules THP-1, cellules U-251, tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules COLO 320,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Informations sur le produit

28494-1-AP cible CRBN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRBN Protéine recombinante Ag29353
• Nom complet: cereblon
• Masse moléculaire calculée: 442 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC017419
• Symbole du gène: CRBN
• Identification du gène (NCBI): 51185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

Publications

Species	Application	Title
human	IHC	Leukemia; Phase 2 study of oral thalidomide-cyclophosphamide-dexamethasone for recurrent/refractory adult Langerhans cell histiocytosis.; Authors - Ji-Nuo Wang
human	WB,IF,IP	Hum Mol Genet; Cereblon suppresses the formation of pathogenic protein aggregates in a p62-dependent manner.; Authors - Liang Zhou ; KD Validated
	WB	J Med Chem; Discovery of Novel Proteolysis-Targeting Chimera Molecules as Degraders of Programmed Cell Death-Ligand 1 for Breast Cancer Therapy; Authors - Hongjia Zhang ; KD Validated
human	WB	Cell Death Discov; Arginine methylation-dependent TRIM47 stability mediated by CARM1 promotes the metastasis of hepatocellular carcinoma; Authors - Yuzhe Tang
human,mouse	WB	Acta Pharm Sin B; Discovery of a potent PROTAC degrader for RNA demethylase FTO as antileukemic therapy; Authors - Lu Liu
human	WB,IP	Bioorg Chem; Discovery of novel 20S proteasome subunit β5 PROTAC degraders as potential therapeutics for pharyngeal carcinoma and Bortezomib-resistant multiple myeloma; Authors - Shumei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-06-2023)

The antibody detected a band around 50 kD at dilution of 1:6000 incubated at room temperature for 1 hour followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: HEK293, A549, H460, HeLa, MCF7

CRBN Antibody Western Blot validation (1:6000 dilution) in HEK293, A549, H460, HeLa, MCF7 (Cat no:28494-1-AP)

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