• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-CREB1

CREB1 Polyclonal Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-12208

Synonymes

CREB, CREB 1, CREB1



Applications testées

Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-12208 cible CREB1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat, singe, souris

Réactivité Humain, rat, singe, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CREB1 Protéine recombinante Ag2852
Nom complet cAMP responsive element binding protein 1
Masse moléculaire calculée 341 aa, 35 kDa
Poids moléculaire observé 43-46 kDa
Numéro d’acquisition GenBankBC010636
Symbole du gène CREB1
Identification du gène (NCBI) 1385
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 CREB1 antibody CL647-12208Download protocol
Standard Protocols
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