Anticorps Polyclonal de lapin anti-CREB3

• Phare
• Validé par KD/KO

CREB3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11275-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 11275-1-AP (same clone as 11275-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11275-1-AP (CREB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11275-1-AP (same clone as 11275-1-PBS)

  WB result of CREB3 antibody (11275-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB3 transfected HeLa cells. This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11275-1-AP (same clone as 11275-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11275-1-AP (CREB3 antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 11275-1-AP (same clone as 11275-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CREB3 antibody (11275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11275-1-PBS cible CREB3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CREB3 Protéine recombinante Ag1823
• Nom complet: cAMP responsive element binding protein 3
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 40-64 kDa
• Numéro d’acquisition GenBank: BC009402
• Symbole du gène: CREB3
• Identification du gène (NCBI): 10488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

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