Anticorps Monoclonal anti-CREB3

CREB3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C12H11

N° de cat : 68379-1-PBS

Synonymes

Leucine zipper protein, Cyclic AMP-responsive element-binding protein 3, CREB-3, CREB 3, cAMP-responsive element-binding protein 3

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Galerie de données de validation

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  WB analysis of T-47D using 68379-1-Ig (same clone as 68379-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50828-4

  Cytometric bead array standard curve of MP50828-4, CREB3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68379-7-PBS. Detection antibody: 68379-1-PBS. Standard:Ag1823. Range: 1.563-100 ng/mL.

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Informations sur le produit

68379-1-PBS cible CREB3 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CREB3 Protéine recombinante Ag1823
• Nom complet: cAMP responsive element binding protein 3
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 40-64 kDa
• Numéro d’acquisition GenBank: BC009402
• Symbole du gène: CREB3
• Identification du gène (NCBI): 10488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

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