Anticorps Monoclonal anti-CREB3L1,OASIS

CREB3L1,OASIS Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B5B4

N° de cat : 67617-1-PBS

Synonymes

CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1

---

Galerie de données de validation

• 

  WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67617-1-Ig (same clone as 67617-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67617-1-Ig (same clone as 67617-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67617-1-Ig (same clone as 67617-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67617-1-Ig (same clone as 67617-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67617-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50829-2

  Cytometric bead array standard curve of MP50829-2, CREB3L1,OASIS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67617-1-PBS. Detection antibody: 67617-4-PBS. Standard:Ag28042. Range: 1.563-100 ng/mL.

---

Informations sur le produit

67617-1-PBS cible CREB3L1,OASIS dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CREB3L1,OASIS Protéine recombinante Ag28042
• Nom complet: cAMP responsive element binding protein 3-like 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC015781
• Symbole du gène: CREB3L1/OASIS
• Identification du gène (NCBI): 90993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

{{ptg:RelatedPrimaryAntibodies}}