Anticorps Polyclonal de lapin anti-CRELD1

CRELD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29903-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of rat lung using 29903-1-AP (same clone as 29903-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 29903-1-AP (same clone as 29903-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 29903-1-AP (same clone as 29903-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 29903-1-AP (same clone as 29903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29903-1-AP (same clone as 29903-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29903-1-PBS cible CRELD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRELD1 Protéine recombinante Ag31593
• Nom complet: cysteine-rich with EGF-like domains 1
• Masse moléculaire calculée: 45KD
• Poids moléculaire observé: 45 kDa, 70-75 kDa
• Numéro d’acquisition GenBank: NM_001031717
• Symbole du gène: CRELD1
• Identification du gène (NCBI): 78987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).

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