Anticorps Monoclonal anti-CREST

CREST Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D5D10

N° de cat : 60314-1-PBS

Synonymes

SS18L1, 3D5D10, Calcium-responsive transactivator, KIAA0693, LP2261

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 60314-1-Ig (same clone as 60314-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60314-1-Ig (same clone as 60314-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60314-1-Ig (same clone as 60314-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60314-1-Ig (same clone as 60314-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 60314-1-Ig (same clone as 60314-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51462-1

  Cytometric bead array sample test of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS.

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  Cytometric bead array standard curve of MP51462-1

  Cytometric bead array standard curve of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS. Standard:Ag3119. Range: 0.781-100 ng/mL.

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Informations sur le produit

60314-1-PBS cible CREST dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CREST Protéine recombinante Ag3119
• Nom complet: synovial sarcoma translocation gene on chromosome 18-like 1
• Masse moléculaire calculée: 396 aa, 43 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC034494
• Symbole du gène: CREST
• Identification du gène (NCBI): 26039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).

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