Anticorps Polyclonal de lapin anti-CRIP1

• Phare
• Validé par KD/KO

CRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 15349-1-AP

Synonymes

CRP-1, CRP1, CRP 1, CRIP 1, CRIP

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Galerie de données de validation

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  WB analysis of HeLa using 15349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15349-1-AP (CRIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15349-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15349-1-AP (CRIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15349-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15349-1-AP (CRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15349-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15349-1-AP (CRIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15349-1-AP cible CRIP1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRIP1 Protéine recombinante Ag7588
• Nom complet: cysteine-rich protein 1 (intestinal)
• Masse moléculaire calculée: 9 kDa
• Poids moléculaire observé: 8-9 kDa
• Numéro d’acquisition GenBank: BC002738
• Symbole du gène: CRIP1
• Identification du gène (NCBI): 1396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).

Publications

Species	Application	Title
human	WB,CoIP	EBioMedicine; CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy; Authors - Peixia Tang ; KD Validated
human	WB	Dis Markers; Cysteine-Rich Intestinal Protein 1 Served as an Epithelial Ovarian Cancer Marker via Promoting Wnt/β-Catenin-Mediated EMT and Tumour Metastasis.; Authors - Yujuan Liu ; KD Validated
human	WB,CoIP	Leuk Res; CRIP1 supports the growth and migration of AML-M5 subtype cells by activating Wnt/β-catenin pathway; Authors - Xiaoling Deng ; KD Validated
human	IHC,IP	EMBO J; CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma; Authors - Jing Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

I labeled the 50 kD mark. CRIP1 should be a small protein ~8-9 kD. The blot was exposed for 120s and there were no bands with low molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CRIP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15349-1-AP)

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