Anticorps Polyclonal de lapin anti-CRIP2

CRIP2 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 14801-1-AP

Synonymes

CRIP, CRIP2, CRP 2, CRP2, cysteine rich protein 2, ESP1, Protein ESP1

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Galerie de données de validation

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  WB analysis of mouse heart using 14801-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14801-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14801-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14801-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14801-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14801-1-AP

  Immunofluorescent analysis of HeLa cells using 14801-1-AP (CRIP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, cellules MCF-7, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14801-1-AP cible CRIP2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRIP2 Protéine recombinante Ag6515
• Nom complet: cysteine-rich protein 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 23-29 kDa
• Numéro d’acquisition GenBank: BC000434
• Symbole du gène: CRIP2
• Identification du gène (NCBI): 1397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB,IF,IP	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-07-2019)

The CRIP2 antibody works well and the protein shows up prominently as the lower band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CRIP2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14801-1-AP)