Anticorps Polyclonal de lapin anti-CRIPT

• Phare
• Validé par KD/KO

CRIPT Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11211-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

• 

  IP experiment of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  IP result of anti-CRIPT (IP:11211-1-AP, 4ug; Detection:11211-1-AP 1:500) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 11211-1-AP (same clone as 11211-1-PBS)

  Immunofluorescent analysis of L02 cells using 11211-1-AP (CRIPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11211-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11211-1-PBS cible CRIPT dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRIPT Protéine recombinante Ag1708
• Nom complet: cysteine-rich PDZ-binding protein
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC018653
• Symbole du gène: CRIPT
• Identification du gène (NCBI): 9419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains

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