Anticorps Polyclonal de lapin anti-CRISP3

• Phare
• Validé par KD/KO

CRISP3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14847-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14847-1-AP (same clone as 14847-1-PBS)

  human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 14847-1-AP (same clone as 14847-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14847-1-AP (same clone as 14847-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  IP experiment of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

  IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14847-1-PBS cible CRISP3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cysteine-rich secretory protein 3
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: NM_006061
• Symbole du gène: CRISP3
• Identification du gène (NCBI): 10321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

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