Anticorps Recombinant de lapin anti-CDK12/CRKRS

CDK12/CRKRS Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250880E5

N° de cat : 86296-3-PBS

Synonymes

CrkRS, CDC2 related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, CDC2-related protein kinase 7, CDK12

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Galerie de données de validation

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  WB analysis using 86296-3-RR (same clone as 86296-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86296-3-RR (same clone as 86296-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86296-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86296-3-RR against Human CDK12/CRKRS were performed. The affinity constant is 0.106 nM.

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Informations sur le produit

86296-3-PBS cible CDK12/CRKRS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK12/CRKRS Protéine recombinante Ag25312
• Nom complet: Cdc2-related kinase, arginine/serine-rich
• Masse moléculaire calculée: 1490 aa, 164 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC150265
• Symbole du gène: CDK12
• Identification du gène (NCBI): 51755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).

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