Anticorps Monoclonal anti-CRM1

• Phare
• Validé par KD/KO

CRM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2D3B6

N° de cat : 66763-1-PBS

Synonymes

XPO1, 2D3B6, Exp1, Exportin 1, Exportin-1

---

Galerie de données de validation

• 

  WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66763-1-Ig (same clone as 66763-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66763-1-Ig (same clone as 66763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66763-1-Ig (same clone as 66763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66763-1-PBS in a different storage buffer formulation.

---

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66763-1-PBS cible CRM1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CRM1 Protéine recombinante Ag27337
• Nom complet: exportin 1 (CRM1 homolog, yeast)
• Masse moléculaire calculée: 1071 aa, 123 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC032847
• Symbole du gène: CRM1
• Identification du gène (NCBI): 7514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publications

Species	Application	Title
human	IHC	Adv Sci (Weinh); Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer; Authors - Yu Tang

{{ptg:RelatedPrimaryAntibodies}}