Anticorps Polyclonal de lapin anti-CRMP2

CRMP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14686-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14686-1-AP (same clone as 14686-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 14686-1-AP (CRMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14686-1-AP (same clone as 14686-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CRMP2 antibody (14686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14686-1-PBS cible CRMP2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRMP2 Protéine recombinante Ag5985
• Nom complet: dihydropyrimidinase-like 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC056408
• Symbole du gène: CRMP2
• Identification du gène (NCBI): 1808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

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