Anticorps Polyclonal de lapin anti-CROP

• Phare
• Validé par KD/KO

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14504-1-PBS

Synonymes

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

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Galerie de données de validation

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  WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14504-1-AP (same clone as 14504-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14504-1-PBS cible CROP dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CROP Protéine recombinante Ag5961
• Nom complet: cisplatin resistance-associated overexpressed protein
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC056409
• Symbole du gène: CROP
• Identification du gène (NCBI): 51747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

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