Anticorps Recombinant de lapin anti-CROP

CROP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251672C12

N° de cat : 86690-1-RR

Synonymes

cAMP regulatory element-associated protein 1, Cisplatin resistance-associated-overexpressed protein, CRE associated protein 1, CREAP 1, CREAP1

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Galerie de données de validation

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  WB analysis using 86690-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86690-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86690-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (86690-1-RR, Clone: 251672C12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86690-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86690-1-RR against Human CROP were performed. The affinity constant is 3.07 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86690-1-RR cible CROP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CROP Protéine recombinante Ag5961
• Nom complet: cisplatin resistance-associated overexpressed protein
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC056409
• Symbole du gène: CROP
• Identification du gène (NCBI): 51747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

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