Anticorps Recombinant de lapin anti-CRTAM/CD355

CRTAM/CD355 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241280C4

N° de cat : 84068-4-PBS

Synonymes

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280C4

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Galerie de données de validation

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  WB analysis using 84068-4-RR (same clone as 84068-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84068-4-RR (CRTAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84068-4-RR (same clone as 84068-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84068-4-RR (CRTAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84068-4-RR (same clone as 84068-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRTAM antibody (84068-4-RR, Clone: 241280C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84068-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84068-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84068-4-RR against Human CRTAM were performed. The affinity constant is 0.427 nM.

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Informations sur le produit

84068-4-PBS cible CRTAM/CD355 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cytotoxic and regulatory T cell molecule
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_019604.4
• Symbole du gène: CRTAM
• Identification du gène (NCBI): 56253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).

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