Anticorps Recombinant de lapin anti-CRTAM/CD355

CRTAM/CD355 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241280B12

N° de cat : 98157-1-PBS

Synonymes

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280B12

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Galerie de données de validation

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  FC experiment of human PBMCs using 98157-1-RR (same clone as 98157-1-PBS)

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8. Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98157-1-RR (same clone as 98157-1-PBS)

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG (dashed, black). Cells were not fixed. This data was developed using the same antibody clone with 98157-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98157-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98157-1-RR against Human CRTAM/CD355 were performed. The affinity constant is 0.449 nM.

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Informations sur le produit

98157-1-PBS cible CRTAM/CD355 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cytotoxic and regulatory T cell molecule
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: NM_019604.4
• Symbole du gène: CRTAM
• Identification du gène (NCBI): 56253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).

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