Anticorps Recombinant de lapin anti-CRTAM/CD355

CRTAM/CD355 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241280B12

N° de cat : 98157-1-RR

Synonymes

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355, 241280B12

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Galerie de données de validation

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  FC experiment of human PBMCs using 98157-1-RR

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8. Cells were not fixed.

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  FC experiment of human PBMCs using 98157-1-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CRTAM/CD355 Rabbit Recombinant Antibody (98157-1-RR, Clone: 241280B12) and PE-conjugated Goat Anti-Rabbit IgG (dashed, black). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98157-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98157-1-RR against Human CRTAM/CD355 were performed. The affinity constant is 0.449 nM.

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98157-1-RR cible CRTAM/CD355 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cytotoxic and regulatory T cell molecule
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: NM_019604.4
• Symbole du gène: CRTAM
• Identification du gène (NCBI): 56253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).

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