Anticorps Recombinant de lapin anti-CRTAM/CD355

CRTAM/CD355 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241280B12

N° de cat : CL647-98157

Synonymes

CRTAM, 241280B12, CD355, Class-I MHC-restricted T-cell-associated molecule, Cytotoxic and regulatory T-cell molecule

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98157

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD8 and 5 ul CoraLite® Plus 647 Anti-Human CRTAM/CD355 Rabbit RecAb (CL647-98157, Clone: 241280B12). Cells were not fixed.

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  FC experiment of human PBMCs using CL647-98157

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CRTAM/CD355 Rabbit RecAb (CL647-98157, Clone: 241280B12) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CRTAM/CD355 Rabbit RecAb (CL647-98157, Clone: 241280B12) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98157 cible CRTAM/CD355 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CRTAM/CD355 Protéine recombinante Eg1541
• Nom complet: cytotoxic and regulatory T cell molecule
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: NM_019604.4
• Symbole du gène: CRTAM
• Identification du gène (NCBI): 56253
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223).

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