Anticorps Polyclonal de lapin anti-CRTC2,TORC2

• Phare
• Validé par KD/KO

CRTC2,TORC2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 12497-1-AP

Synonymes

CRTC2, CRTC2,TORC2, RP11 422P24.6, TORC 2, TORC2, Transducer of CREB protein 2

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Galerie de données de validation

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  WB analysis using 12497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12497-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse kidney using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 12497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12497-1-AP

  Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 12497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, rein de rat, rein de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu de carcinome urothélial humain, tissu de gliome humain, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12497-1-AP cible CRTC2,TORC2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRTC2,TORC2 Protéine recombinante Ag3167
• Nom complet: CREB regulated transcription coactivator 2
• Masse moléculaire calculée: 693 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC053562
• Symbole du gène: CRTC2
• Identification du gène (NCBI): 200186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Adv Sci (Weinh); cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.; Authors - Zeyun Mi ; KD Validated
mouse	WB	Mol Cell; LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation; Authors - Shelby E Compton
human	IHC	Am J Transplant; The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside.; Authors - Qi Ling
mouse	IHC,IF	J Neurosci; Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock.; Authors - Sakamoto Kensuke K
human,mouse,bovine	WB,IF	J Neurosci; CREB Coactivator CRTC2 Plays a Crucial Role in Endothelial Function.; Authors - Hideaki Kanki
human	WB,IF	J Virol; Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells.; Authors - Yuan Jinxiang J

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