Anticorps Polyclonal de lapin anti-TORC2

• Phare
• Validé par KD/KO

TORC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12497-1-PBS

Synonymes

CRTC2,TORC2, CRTC2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2, TORC 2

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Galerie de données de validation

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  WB analysis using 12497-1-AP (same clone as 12497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12497-1-AP (same clone as 12497-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12497-1-AP (same clone as 12497-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12497-1-AP (same clone as 12497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12497-1-AP (same clone as 12497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CRTC2,TORC2 antibody (12497-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12497-1-AP (same clone as 12497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12497-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12497-1-PBS cible TORC2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TORC2 Protéine recombinante Ag3167
• Nom complet: CREB regulated transcription coactivator 2
• Masse moléculaire calculée: 693 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC053562
• Symbole du gène: TORC2
• Identification du gène (NCBI): 200186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

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