Anticorps Monoclonal anti-CRYBB1

CRYBB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

6F12B12

N° de cat : 60273-1-Ig

Synonymes

6F12B12, Beta B1 crystallin, Beta crystallin B1, Beta-B1 crystallin, Beta-crystallin B1

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Galerie de données de validation

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  WB analysis of rabbit eye using 60273-1-Ig

  rabbit eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat eye using 60273-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 60273-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 60273-1-Ig

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 60273-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu oculaire de rat, cellules C6, tissu oculaire de souris
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60273-1-Ig cible CRYBB1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CRYBB1 Protéine recombinante Ag4722
• Nom complet: crystallin, beta B1
• Masse moléculaire calculée: 252 aa, 28 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC036790
• Symbole du gène: CRYBB1
• Identification du gène (NCBI): 1414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.

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