Anticorps Polyclonal de lapin anti-CSDA

• Phare
• Validé par KD/KO

CSDA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27785-1-PBS

Synonymes

cold shock domain protein A, Cold shock domain-containing protein A, DBPA, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB

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Galerie de données de validation

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  WB analysis of KYSE-30 using 27785-1-AP (same clone as 27785-1-PBS)

  WB result of CSDA antibody (27785-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  WB analysis using 27785-1-AP (same clone as 27785-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27785-1-AP (same clone as 27785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 27785-1-AP (same clone as 27785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 27785-1-AP (same clone as 27785-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSDA antibody (27785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27785-1-PBS cible CSDA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSDA Protéine recombinante Ag26839
• Nom complet: cold shock domain protein A
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 60-68 kDa
• Numéro d’acquisition GenBank: BC008801
• Symbole du gène: CSDA
• Identification du gène (NCBI): 8531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

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