Anticorps Recombinant de lapin anti-CSDC2

CSDC2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241031A11

N° de cat : 83886-6-RR

Synonymes

RNA-binding protein PIPPin, RNA binding protein PIPPin, PIPPIN, Cold shock domain-containing protein C2, 241031A11

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Galerie de données de validation

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  IF Staining of HeLa using 83886-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSDC2 antibody (83886-6-RR, Clone: 241031A11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-2 OS using 83886-6-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug CSDC2 Recombinant antibody (83886-6-RR, Clone:241031A11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83886-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83886-6-RR against Human CSDC2 were performed. The affinity constant is 0.256 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): U-2 OS,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83886-6-RR cible CSDC2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CSDC2 Protéine recombinante Ag16216
• Nom complet: cold shock domain containing C2, RNA binding
• Masse moléculaire calculée: 153 aa, 17 kDa
• Numéro d’acquisition GenBank: BC067113
• Symbole du gène: CSDC2
• Identification du gène (NCBI): 27254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIPPIN, also known as CSDC2 (Cold shock domain-containing protein C2) is an RNA‐binding protein (RBP), highly enriched in the rat brain, specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum (PMID: 10446180). PIPPIN has the potential to undergo different posttranslational modifications and might be a good candidate to regulate the synthesis of specific proteins in response to extracellular stimuli. Nuclear CSDC2 is connected to proliferation and cytoplasmic CSDC2 to terminal differentiation in the decidua and that CSDC2 could regulate differentiation during decidua development (PMID: 30078185, PMID: 17053029).

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