Anticorps Polyclonal de lapin anti-CSDE1

• Phare
• Validé par KD/KO

CSDE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 13319-1-PBS

Synonymes

UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1

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Galerie de données de validation

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  WB analysis using 13319-1-AP (same clone as 13319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13319-1-AP (CSDE1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 13319-1-AP (same clone as 13319-1-PBS)

  WB result of CSDE1 antibody (13319-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSDE1 transfected A431 cells. This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13319-1-AP (same clone as 13319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13319-1-AP (CSDE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13319-1-AP (same clone as 13319-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSDE1 antibody (13319-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13319-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13319-1-PBS cible CSDE1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSDE1 Protéine recombinante Ag4173
• Nom complet: cold shock domain containing E1, RNA-binding
• Masse moléculaire calculée: 798 aa, 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC032446
• Symbole du gène: CSDE1
• Identification du gène (NCBI): 7812
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.

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