Anticorps Monoclonal anti-Gamma Cystathionase

• Phare
• Validé par KD/KO

Gamma Cystathionase Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

CloneNo.

2C7F9

N° de cat : 60234-1-Ig

Synonymes

CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase

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Galerie de données de validation

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  WB analysis using 60234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma Cystathionase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of PC-3 cells using 60234-1-Ig

  WB result of CTH antibody (60234-1-Ig, 1:500) with si-control and si-CTH transfected PC-3 cells.

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  WB analysis of HEK-293 using 60234-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60234-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60234-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60234-1-Ig (Gamma cystathionase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50.

• 

  IHC staining of human liver cancer using 60234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 60234-1-Ig (Gamma cystathionase antibody at dilution of 1:50.

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  IF Staining of HepG2 using 60234-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60234-1-Ig(Gamma cystathionase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules K-562, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

60234-1-Ig cible Gamma Cystathionase dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Gamma Cystathionase Protéine recombinante Ag2872
• Nom complet: cystathionase (cystathionine gamma-lyase)
• Masse moléculaire calculée: 405 aa, 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC015807
• Symbole du gène: CTH
• Identification du gène (NCBI): 1491
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309).

Publications

Species	Application	Title
human	WB	Sci Adv; Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.; Authors - Anne von Mässenhausen
human	WB	Autophagy; Vascular smooth muscle cell-derived hydrogen sulfide promotes atherosclerotic plaque stability via TFEB (transcription factor EB)-mediated autophagy.; Authors - Zhenzhen Chen
rat	WB,IF	Oxid Med Cell Longev; Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia.; Authors - Liren Zhong
human	WB	Int J Mol Sci; Fructose Intake Impairs the Synergistic Vasomotor Manifestation of Nitric Oxide and Hydrogen Sulfide in Rat Aorta.; Authors - Andrea Berenyiova
rat	WB	Int J Mol Sci; Vasoactive Effects of Chronic Treatment with Fructose and Slow-Releasing H2S Donor GYY-4137 in Spontaneously Hypertensive Rats: The Role of Nitroso and Sulfide Signalization; Authors - Andrea Berenyiova
rat	WB	Pharmacol Res; The novel H2S-donor 4-carboxyphenyl isothiocyanate promotes cardioprotective effects against ischemia/reperfusion injury through activation of mitoKATP channels and reduction of oxidative stress.; Authors - Lara Testai