Anticorps Polyclonal de lapin anti-CSE1L

• Phare
• Validé par KD/KO

CSE1L Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22219-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22219-1-AP (same clone as 22219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22219-1-AP (same clone as 22219-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW264.7 using 22219-1-AP (same clone as 22219-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 22219-1-AP (same clone as 22219-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22219-1-AP (CSE1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 22219-1-AP (same clone as 22219-1-PBS)

  IP result of anti-CSE1L (IP:22219-1-AP, 4ug; Detection:22219-1-AP 1:2000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 22219-1-AP (same clone as 22219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22219-1-AP (CSE1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22219-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22219-1-PBS cible CSE1L dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSE1L Protéine recombinante Ag17556
• Nom complet: CSE1 chromosome segregation 1-like (yeast)
• Masse moléculaire calculée: 971 aa, 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC109313
• Symbole du gène: CSE1L
• Identification du gène (NCBI): 1434
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSE1L (Chromosome Segregation 1-Like) is located on 20q13.13, and encodes a 971-amino-acid protein. It associates with microtubules and mitotic spindles, and is also considered to have a role in tumor progression. The CSE1L protein includes a nuclear localization signal allowing for transport into the nucleus via the importin-alpha/beta heterodimer. In addition, CSE1L plays roles in cell proliferation and apoptosis.

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