Anticorps Polyclonal de lapin anti-CSF1R

CSF1R Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 25949-1-AP

Synonymes

C FMS, CD115, CSF 1 R, CSF1R, CSFR, FIM2, FMS, Proto oncogene c Fms

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Galerie de données de validation

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  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

25949-1-AP cible CSF1R dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSF1R Protéine recombinante Ag22845
• Nom complet: colony stimulating factor 1 receptor
• Masse moléculaire calculée: 972 aa, 108 kDa
• Numéro d’acquisition GenBank: BC047521
• Symbole du gène: CSF1R
• Identification du gène (NCBI): 1436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Targeting CSF1R Alone or in Combination with PD1 in Experimental Glioma.; Authors - Justyna M Przystal
human	IHC	Arthritis Res Ther; Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.; Authors - Ping Li
human	IHC	CNS Neurosci Ther; A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma; Authors - Wenhua Fan
mouse	WB	Exploration (Beijing); Microglial targeted therapy relieves cognitive impairment caused by Cntnap4 deficiency; Authors - Wenlong Zhang
human	IHC	Front Genet; Human placenta-based genome-wide mRNA sequencing to identify TEK/IGF1/CSF1/ANGPT2 as crucial segments in the pathogenesis of pre-eclampsia; Authors - Lifeng Wang
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Used in synovium PFA-fixed slides to detect macrophages. Works well.

• Applications: Immunohistochemistry