Anticorps Polyclonal de lapin anti-CSF1R/CD115

CSF1R/CD115 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 25949-1-AP

Synonymes

CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor

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Galerie de données de validation

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  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25949-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of THP-1 using 25949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF1R/CD115 antibody (25949-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

25949-1-AP cible CSF1R/CD115 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSF1R/CD115 Protéine recombinante Ag22845
• Nom complet: colony stimulating factor 1 receptor
• Masse moléculaire calculée: 972 aa, 108 kDa
• Numéro d’acquisition GenBank: BC047521
• Symbole du gène: CSF1R/CD115
• Identification du gène (NCBI): 1436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Targeting CSF1R Alone or in Combination with PD1 in Experimental Glioma.; Authors - Justyna M Przystal
human	IHC	Arthritis Res Ther; Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.; Authors - Ping Li
human	IHC	CNS Neurosci Ther; A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma; Authors - Wenhua Fan
rat	WB	J Nat Med; Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology; Authors - Xin-Hua Zhu
mouse	WB	J Transl Med; ITIH5-mediated fibroblast/macrophage crosstalk exacerbates cardiac remodelling after myocardial infarction; Authors - Yirong Wu
mouse	WB	Toxics; CuO-NPs Induce Apoptosis and Functional Impairment in BV2 Cells Through the CSF-1R/PLCγ2/ERK/Nrf2 Pathway; Authors - Linhui Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Used in synovium PFA-fixed slides to detect macrophages. Works well.

• Applications: Immunohistochemistry

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