Anticorps Monoclonal anti-CSK

CSK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B12C5

N° de cat : 66115-1-PBS

Synonymes

Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase

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Galerie de données de validation

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  WB analysis of Jurkat using 66115-1-Ig (same clone as 66115-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66115-1-Ig (same clone as 66115-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66115-1-Ig (same clone as 66115-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66115-1-Ig (same clone as 66115-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66115-1-Ig (same clone as 66115-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSK antibody (66115-1-Ig, Clone: 3B12C5 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66115-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66115-1-PBS cible CSK dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CSK Protéine recombinante Ag12103
• Nom complet: c-src tyrosine kinase
• Masse moléculaire calculée: 450 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC106073
• Symbole du gène: CSK
• Identification du gène (NCBI): 1445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

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