Anticorps Monoclonal anti-CSN2

CSN2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H10E6

N° de cat : 66435-1-PBS

Synonymes

ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 66435-1-Ig (same clone as 66435-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66435-1-Ig (same clone as 66435-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66435-1-Ig (same clone as 66435-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66435-1-Ig (same clone as 66435-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (CSN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (COPS2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human ovary tumor using 66435-1-Ig (same clone as 66435-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CSN2 antibody (66435-1-Ig, Clone: 2H10E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66435-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66435-1-PBS cible CSN2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CSN2 Protéine recombinante Ag1415
• Nom complet: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC012629
• Symbole du gène: COPS2
• Identification du gène (NCBI): 9318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

{{ptg:RelatedPrimaryAntibodies}}