Anticorps Polyclonal de lapin anti-CSNK1G1

CSNK1G1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 16384-1-AP

Synonymes

casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1

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Galerie de données de validation

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  WB analysis using 16384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16384-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16384-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16384-1-AP

  IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug.

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  IHC staining of human liver using 16384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

16384-1-AP cible CSNK1G1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK1G1 Protéine recombinante Ag9395
• Nom complet: casein kinase 1, gamma 1
• Masse moléculaire calculée: 422 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017236
• Symbole du gène: CSNK1G1
• Identification du gène (NCBI): 53944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

Publications

Species	Application	Title
human	WB,IP	J Immunol; Casein Kinase 1γ1 Inhibits the RIG-I/TLR Signaling Pathway through Phosphorylating p65 and Promoting Its Degradation.; Authors - Yetao Wang
human	WB	Leukemia; A highly selective and orally bioavailable casein kinase 1 alpha degrader through p53 signaling pathway targets B-cell lymphoma cells; Authors - Shi Feng

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