Anticorps Polyclonal de lapin anti-CSNK1G1

CSNK1G1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16384-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16384-1-AP (same clone as 16384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 16384-1-AP (same clone as 16384-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

  IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16384-1-PBS cible CSNK1G1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK1G1 Protéine recombinante Ag9395
• Nom complet: casein kinase 1, gamma 1
• Masse moléculaire calculée: 422 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017236
• Symbole du gène: CSNK1G1
• Identification du gène (NCBI): 53944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

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