Anticorps Monoclonal anti-CSNK2A1

• Phare
• Validé par KD/KO

CSNK2A1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5E8

N° de cat : 68200-1-PBS

Synonymes

1D5E8, CK II alpha, CK2a, CK2A1, CK2α

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Galerie de données de validation

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  WB analysis using 68200-1-Ig (same clone as 68200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68200-1-Ig (same clone as 68200-1-PBS)

  WB result of CSNK2A1 antibody (68200-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit heart using 68200-1-Ig (same clone as 68200-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68200-1-Ig (CSNK2A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68200-1-Ig (same clone as 68200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A1 antibody (68200-1-Ig, Clone: 1D5E8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68200-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68200-1-PBS cible CSNK2A1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CSNK2A1 Protéine recombinante Ag29062
• Nom complet: casein kinase 2, alpha 1 polypeptide
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC011668
• Symbole du gène: CSNK2A1
• Identification du gène (NCBI): 1457
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

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