Anticorps Polyclonal de lapin anti-CSNK2A2

CSNK2A2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10606-1-AP

Synonymes

Casein kinase II subunit alpha', CK II alpha', CK2A2, CSNK2A1, CSNK2A2

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Galerie de données de validation

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  WB analysis of HepG2 using 10606-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10606-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 10606-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10606-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10606-1-AP

  IP result of anti-CSNK2A2 (IP:10606-1-AP, 4ug; Detection:10606-1-AP 1:1000) with Jurkat cells lysate 2800 ug.

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  IF Staining of HepG2 using 10606-1-AP

  Immunofluorescent analysis of HepG2 cells using 10606-1-AP (CSNK2A2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HT-1080, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

10606-1-AP cible CSNK2A2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK2A2 Protéine recombinante Ag0877
• Nom complet: casein kinase 2, alpha prime polypeptide
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC008812
• Symbole du gène: CSNK2A2
• Identification du gène (NCBI): 1459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).

Publications

Species	Application	Title
mouse	WB,IHC	Acta Neuropathol Commun; CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease.; Authors - Dahyun Yu
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
mouse	WB	Cell Rep; Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease; Authors - Rachel H Mansky ; KD Validated