Anticorps Polyclonal de lapin anti-CSNK2A2

CSNK2A2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 10606-1-AP

Synonymes

CSNK2A 2, CSNK2A1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 10606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10606-1-AP (CSNK2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10606-1-AP

  IP result of anti-CSNK2A2 (IP:10606-1-AP, 4ug; Detection:10606-1-AP 1:8000) with Jurkat cells lysate 1400 ug.

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  IF Staining of HepG2 using 10606-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CSNK2A2 antibody (10606-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10606-1-AP cible CSNK2A2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSNK2A2 Protéine recombinante Ag0877
• Nom complet: casein kinase 2, alpha prime polypeptide
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC008812
• Symbole du gène: CSNK2A2
• Identification du gène (NCBI): 1459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).

Publications

Species	Application	Title
mouse	WB,IHC	Acta Neuropathol Commun; CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease.; Authors - Dahyun Yu
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
mouse	WB	Cell Rep; Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease; Authors - Rachel H Mansky ; KD Validated
human	WB,IP	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated
human	WB	Int Immunopharmacol; A quinolinyl resveratrol derivative alleviates acute ischemic stroke injury by promoting mitophagy for neuroprotection via targeting CK2α'; Authors - Libin Xu

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