Anticorps Monoclonal anti-CSNK2B

• Phare
• Validé par KD/KO

CSNK2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5A6

N° de cat : 67866-1-PBS

Synonymes

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

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Galerie de données de validation

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  WB analysis using 67866-1-Ig (same clone as 67866-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67866-1-Ig (same clone as 67866-1-PBS)

  WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells. This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67866-1-Ig (same clone as 67866-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67866-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67866-1-PBS cible CSNK2B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CSNK2B Protéine recombinante Ag19180
• Nom complet: casein kinase 2, beta polypeptide
• Masse moléculaire calculée: 215 aa, 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC112017
• Symbole du gène: CSNK2B
• Identification du gène (NCBI): 1460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

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