Anticorps Polyclonal de lapin anti-CSNK2B

• Phare
• Validé par KD/KO

CSNK2B Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20234-1-PBS

Synonymes

CSNK2B-specific, Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B

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Galerie de données de validation

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  WB analysis using 20234-1-AP (same clone as 20234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20234-1-AP (same clone as 20234-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 20234-1-AP (same clone as 20234-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20234-1-AP (same clone as 20234-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20234-1-AP (same clone as 20234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20234-1-AP (CSNK2B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 20234-1-AP (same clone as 20234-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CSNK2B-specific (20234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20234-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20234-1-PBS cible CSNK2B dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: casein kinase 2, beta polypeptide
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NM_001320
• Symbole du gène: CSNK2B
• Identification du gène (NCBI): 1460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B.

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