Anticorps Polyclonal de lapin anti-CSRP1

CSRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 13432-1-AP

Synonymes

CRP, CRP1, CSRP, CYRP, Cysteine and glycine-rich protein 1

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Galerie de données de validation

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  WB analysis of human stomach using 13432-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 13432-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:300.

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  IHC staining of human colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain
• Résultats positifs en IP: tissu d'estomac de souris,
• Résultats positifs en IHC: human normal colon, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13432-1-AP cible CSRP1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSRP1 Protéine recombinante Ag4236
• Nom complet: cysteine and glycine-rich protein 1
• Masse moléculaire calculée: 193 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC032493
• Symbole du gène: CSRP1
• Identification du gène (NCBI): 1465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CSRP1 (Cysteine and glycine-rich protein 1), designated initially as CRP1, constitutes a cysteine-rich protein derived from placental sources and exhibits a response profile analogous to c-myc. CSRP1 has been recognized as a tumor suppressor in several types of cancers including colorectal cancer, and cholangiocarcinoma (PMID: 37113556). Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia (PMID: 38813484, 40198006).

Publications

Species	Application	Title
mouse	WB,IF	Mol Psychiatry; PD-L1/PD-1 checkpoint pathway regulates astrocyte morphogenesis and myelination during brain development; Authors - Yanyan Wang
rat	WB	Mol Med Rep; Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes.; Authors - Hui Ren

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