Anticorps Polyclonal de lapin anti-CSRP2

• Phare
• Validé par KD/KO

CSRP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10892-2-PBS

Synonymes

CRP2, Cysteine and glycine-rich protein 2, Cysteine rich protein 2, Cysteine-rich protein 2, LMO5

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Galerie de données de validation

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  WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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  WB analysis using 10892-2-AP (same clone as 10892-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 10892-2-AP (same clone as 10892-2-PBS)

  IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10892-2-AP (same clone as 10892-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10892-2-AP (CSRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10892-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10892-2-PBS cible CSRP2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CSRP2 Protéine recombinante Ag1338
• Nom complet: cysteine and glycine-rich protein 2
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000992
• Symbole du gène: CSRP2
• Identification du gène (NCBI): 1466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CSRP2 (Cysteine and glycine-rich protein 2) is a member of the LIM-only family of cysteine-rich proteins and contains two LIM domains, which has been recently implicated in the progression and metastasis of a variety of cancers. CSRP2 is functionally linked to the activity of TGF-b1 in hepatic stellate cells (HSC), derivatives thereof, and in differentiated VSMC.CSRP2 knockdown signifcantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells (PMID: 33042270). CSRP2 is a short (21 kDa) two LIM domain-containing protein, which is upregulated in invasive breast cancer cells, and localizes along the protrusive actin core of invadopodium1 (PMID: 29976963, 16735029).

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