Anticorps Polyclonal de lapin anti-Cystatin SN/CST1

• Phare
• Validé par KD/KO

Cystatin SN/CST1 Polyclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 16025-1-PBS

Synonymes

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

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Galerie de données de validation

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  WB analysis of human saliva using 16025-1-AP (same clone as 16025-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

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  WB analysis using 16025-1-AP (same clone as 16025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 16025-1-AP (same clone as 16025-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

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  WB analysis of human adrenal gland using 16025-1-AP (same clone as 16025-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 16025-1-AP (same clone as 16025-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 16025-1-AP (same clone as 16025-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16025-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16025-1-PBS cible Cystatin SN/CST1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cystatin SN/CST1 Protéine recombinante Ag8862
• Nom complet: cystatin SN
• Masse moléculaire calculée: 141 aa, 16 kDa
• Poids moléculaire observé: 14-16 kDa
• Numéro d’acquisition GenBank: BC021225
• Symbole du gène: CST1
• Identification du gène (NCBI): 1469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions. CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.

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