Anticorps Polyclonal de lapin anti-CST6

• Phare
• Validé par KD/KO

CST6 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17076-1-AP

Synonymes

CST6, Cystatin 6, Cystatin E, cystatin E/M, Cystatin M

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Galerie de données de validation

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  IHC staining of human breast cancer using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 17076-1-AP (CST6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17076-1-AP (CST6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque de souris, tissu cutané de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

17076-1-AP cible CST6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CST6 Protéine recombinante Ag10965
• Nom complet: cystatin E/M
• Masse moléculaire calculée: 149 aa, 17 kDa
• Numéro d’acquisition GenBank: BC031334
• Symbole du gène: CST6
• Identification du gène (NCBI): 1474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Theranostics; CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis; Authors - Xiaoxun Li ; KD Validated
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	IHC	Int J Gen Med; Predictive Values of Homeobox Gene A-Antisense Transcript 3 (HOXA-AS3), Cystatin 6 (CST6), and Chromobox Homolog 4 (CBX4) Expressions in Cancer Tissues for Recurrence of Early Colon Cancer After Surgery; Authors - Xiaopeng Zhu