Anticorps Polyclonal de lapin anti-Cystatin A

• Phare
• Validé par KD/KO

Cystatin A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 15962-1-AP

Synonymes

CSTA, Stefin-A, Cystatin-A, N-terminally processed, Cystatin-A, Cystatin AS

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Galerie de données de validation

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  WB analysis of human saliva using 15962-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15962-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15962-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15962-1-AP (Cystatin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 15962-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15962-1-AP (Cystatin A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15962-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cystatin A antibody (15962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu salivaire humain, cellules A549, cellules NIH/3T3
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15962-1-AP cible Cystatin A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cystatin A Protéine recombinante Ag8733
• Nom complet: cystatin A (stefin A)
• Masse moléculaire calculée: 98 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC010379
• Symbole du gène: Cystatin A
• Identification du gène (NCBI): 1475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes.

Publications

Species	Application	Title
human	IHC	Cancer Res; Lung cancer diagnosis from proteomic analysis of preinvasive lesions.; Authors - Rahman S M Jamshedur SM
mouse	WB,IF	Biochem Biophys Res Commun; CSTA plays a role in osteoclast formation and bone resorption by mediating the DAP12/TREM2 pathway; Authors - Rui Wei ; KD Validated
human,mouse	WB	Cell Rep; TGM1/3-mediated transamidation of Exo70 promotes tumor metastasis upon LKB1 inactivation; Authors - Jihuan Hou

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