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Anticorps Polyclonal de lapin anti-CSTF1

CSTF1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10064-2-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using CSTF1 Polyclonal antibody (10064-2-AP)

    WB analysis using 10064-2-AP (same clone as 10064-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10064-2-AP (CSTF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using CSTF1 Polyclonal antibody (10064-2-AP)

    IP experiment of NIH/3T3 using 10064-2-AP (same clone as 10064-2-PBS)

    IP result of anti-CSTF1 (IP:10064-2-AP, 2ug; Detection:10064-2-AP 1:500) with NIH/3T3 cells lysate 2500ug. This data was developed using the same antibody clone with 10064-2-PBS in a different storage buffer formulation.

Informations sur le produit

10064-2-PBS cible CSTF1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CSTF1 Protéine recombinante Ag0109
Nom complet cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Masse moléculaire calculée 48.3 kDa
Poids moléculaire observé 50 kDa
Numéro d’acquisition GenBankBC001011
Symbole du gène CSTF1
Identification du gène (NCBI) 1477
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.

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