Anticorps Recombinant de lapin anti-CTHRC1

CTHRC1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240442E1

N° de cat : 83629-1-RR

Synonymes

UNQ762/PRO1550, Protein NMTC1, Collagen triple helix repeat-containing protein 1, collagen triple helix repeat containing 1, 240442E1

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Galerie de données de validation

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  WB analysis of A375 using 83629-1-RR

  A375 cells were subjected to SDS PAGE followed by western blot with 83629-1-RR (CTHRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A375 using 83629-1-RR

  1x10^6 A375 cells were intracellularly stained with 0.25 ug CTHRC1 Recombinant antibody (83629-1-RR, Clone:240442E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83629-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83629-1-RR against Human CTHRC1 were performed. The affinity constant is 72.9 pM.

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Applications testées

• Résultats positifs en WB: cellules A375,
• Résultats positifs en FC (Intra): cellules A375,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83629-1-RR cible CTHRC1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CTHRC1 Protéine recombinante Ag9812
• Nom complet: collagen triple helix repeat containing 1
• Masse moléculaire calculée: 243 aa, 26 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC014245
• Symbole du gène: CTHRC1
• Identification du gène (NCBI): 115908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

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