Anticorps Recombinant de lapin anti-CTLA-4/CD152

CTLA-4/CD152 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

PE-Cyanine7 Fluorescent Dye

CloneNo.

240793E8

N° de cat : PY7-98078

Synonymes

CTLA4, 240793E8, CD152, CELIAC3, CTLA 4

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Galerie de données de validation

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  FC experiment of human PBMCs using PY7-98078

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul PE-Cyanine7 Anti-Human CTLA-4/CD152 Rabbit RecAb (PY7-98078, Clone: 240793E8). Cells were not fixed.

• 

  FC experiment of human PBMCs using PY7-98078

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul PE-Cyanine7 Anti-Human CTLA-4/CD152 Rabbit RecAb (PY7-98078, Clone: 240793E8) (red) or unstained (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE-Cyanine7 Anti-Human CTLA-4/CD152 Rabbit RecAb (PY7-98078, Clone: 240793E8) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PY7-98078 cible CTLA-4/CD152 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CTLA-4/CD152 Protéine recombinante Eg0972
• Nom complet: cytotoxic T-lymphocyte-associated protein 4
• Masse moléculaire calculée: 223 aa, 25 kDa
• Numéro d’acquisition GenBank: BC070162
• Symbole du gène: CTLA4
• Identification du gène (NCBI): 1493
• Conjugaison: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.

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