Anticorps Recombinant de lapin anti-CTLA-4/CD152

CTLA-4/CD152 Recombinant Antibody for IHC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, FC

Conjugaison

Non conjugué

CloneNo.

240793E8

N° de cat : 98078-1-RR

Synonymes

CTLA4, 240793E8, CD152, CELIAC3, CTLA 4

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Galerie de données de validation

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  IHC staining of human tonsillitis using 98078-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98078-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

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  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 PHA-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 unstimulated human PBMCs were surface stained with Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (98078-1-RR, Clone: 240793E8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98078-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98078-1-RR cible CTLA-4/CD152 dans les applications de IHC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CTLA-4/CD152 Protéine recombinante Eg0972
• Nom complet: cytotoxic T-lymphocyte-associated protein 4
• Masse moléculaire calculée: 223 aa, 25 kDa
• Numéro d’acquisition GenBank: BC070162
• Symbole du gène: CTLA4
• Identification du gène (NCBI): 1493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.

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