Anticorps Polyclonal de lapin anti-Cathepsin A

Cathepsin A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15020-1-AP

Synonymes

Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, CTSA, GLB2, GSL, Lysosomal protective protein, NGBE, PPCA, PPGB, Protective protein cathepsin A

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Galerie de données de validation

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  WB analysis using 15020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 15020-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules DU 145, cellules PC-3, cellules Sp2/0
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du sein humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

15020-1-AP cible Cathepsin A dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin A Protéine recombinante Ag7073
• Nom complet: cathepsin A
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54-60, 32, 20 kDa
• Numéro d’acquisition GenBank: BC000597
• Symbole du gène: CTSA
• Identification du gène (NCBI): 5476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

Publications

Species	Application	Title
mouse	WB	J Hepatol; SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.; Authors - Yan You
human	IHC	Am J Cancer Res; High cathepsin A protein expression predicts poor prognosis and tumor recurrence of hepatocellular carcinoma patients after curative hepatectomy; Authors - Laibang Luo
mouse	WB	Mol Carcinog; Resistance to abemaciclib is associated with increased metastatic potential and lysosomal protein deregulation in breast cancer cells; Authors - Erin R Scheidemann
human	IHC	Sci Rep; Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.; Authors - Huaxiang Wang
mouse	WB	Front Cell Neurosci; Neu1 Is Released From Activated Microglia, Stimulating Microglial Phagocytosis and Sensitizing Neurons to Glutamate.; Authors - David H Allendorf
mouse	FC	Nature; Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.; Authors - Dali Han